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smc medium  (PromoCell)


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    Structured Review

    PromoCell smc medium
    Smc Medium, supplied by PromoCell, used in various techniques. Bioz Stars score: 95/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smc+growth+medium/Smooth+Muscle+Cell+Growth+Medium+2+SupplementPack/pmc12880921-310-17-19
    Average 95 stars, based on 83 article reviews
    smc medium - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Trimethylamine-N-oxide affects cell type-specific pathways and networks in mouse aorta to promote atherosclerotic plaque vulnerability
    Article Snippet: .. Human immortalized coronary artery SMCs (gift from Dr. Clint Miller, University of Virginia, Charlottesville, VA) and human primary aortic SMCs (ATCC, PCS-100-012) were cultured in SMC growth medium with premixed supplements (PromoCell, Cat# C-22062). .. RAW 264.7 mouse macrophage cells (ATCC, Manassas, VA) were cultured in DMEM medium supplemented with 5% FBS, 100 U/mL penicillin, and 100 ug/mL streptomycin.

    Article Title: A model of guided cell self-organization for rapid and spontaneous formation of functional vessels.
    Article Snippet: .. MATERIALS AND METHODS Cell culture and maintenance HUVECs and SMCs (C-12205 and C-12511, PromoCell) were maintained in endothelial growth medium 2 (EGM2; C-22111, PromoCell) and SMC growth medium (SMCGM2; C-22062, PromoCell), respectively, under water-saturated 5% CO2 atmosphere at 37°C. .. Cells were grown in collagen-I–coated flasks (10190103, Thermo Fisher Scientific) and detached at passage 5 for vesseloid formation.

    Article Title: Atrial natriuretic peptide promotes uterine decidualization and a TRAIL-dependent mechanism in spiral artery remodeling
    Article Snippet: .. Human uterine smooth muscle cells (HUtSMCs) (PromoCell, C-12576) were cultured in SMC growth medium (PromoCell, C-22062) with 1% penicillin. .. Human uterine microvascular endothelial cells (HUtMECs) (PromoCell, C-12296) were cultured in EC growth medium (PromoCell, C-22020).

    Article Title: Inducible Depletion of Calpain-2 Mitigates Abdominal Aortic Aneurysm in Mice
    Article Snippet: Mean fluorescence signals were measured using a microplate fluorescent plate reader (Spectramax M2; MolecularDevices, Sunnyvale, CA) per manufacturer’s instructions. .. Human aortic SMCs were purchased from Promo Cell (Catalog No: C-12533) and cultured in SMC growth medium (Promo Cell, Catalog No: C39262). .. The SMC cell purity was verified using a SMC α-actin antibody (1:100, Abcam catalog No: ab5694) by immunohistochemistry ( ).

    Article Title: Computational Screening Strategy for Drug Repurposing Identified Niclosamide as Inhibitor of Vascular Calcification
    Article Snippet: .. Human coronary artery smooth muscle cells (hSMCs, C-12511, PromoCell) were cultured in SMC growth medium (C-22052, PromoCell) at 37°C with 5% CO 2 . .. Cells were inoculated on 24 or 48 well plates by 1 x 10 6 cells/ml with normal condition medium (NM, DMEM with 4.5 g/L glucose (10569010, Thermo Fisher Scientific containing 10% fetal bovine serum).

    Isolation:

    Article Title: Marker profile for the evaluation of human umbilical artery smooth muscle cell quality obtained by different isolation and culture methods
    Article Snippet: .. Freshly isolated cells were seeded in SMC growth medium (Promocell, Heidelberg, Germany) containing 5 % FBS, epidermal growth factor (0.5 ng/mL), basic fibroblast growth factor (2 ng/mL), insulin (5 μg/mL), HEPES (20 mM, Sigma-Aldrich) and gentamicin-amphotericin (Life Technologies) in six wells plates or T25 flasks (Cellstar 243 ® Greiner bio-one) treated with different protein coatings, which were tested to optimize cell adhesion and maintain population purity. .. Briefly, coating of plates with fibronectin (Sigma-Aldrich) was done at a density of 5 μg/cm 2 ; coated plates were air-dried at RT overnight.

    Incubation:

    Article Title: NO, via its target Cx37, modulates calcium signal propagation selectively at myoendothelial gap junctions
    Article Snippet: .. Afterwards the sections were cut into small pieces (500 μm × 500 μm), put with face down of the luminal side of the vein into 6 cm culture dishes and incubated with SMC-growth medium containing DMEM supplemented with 10 U/ml penicillin, 10 mg/ml streptomycin and 10% (FCS) and smooth muscle cell growth Medium 2, (Promocell) in a ratio 4:1 at 37°C and 5% CO 2 . ..



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